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| Version 2013-2014 |
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| CHIM0687-1 | Introduction to protein NMR
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| Duration : | 10h Th, 10h Mon. WS |
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| Number of credits : |
| Master in Biomedicine, Research Focus, 1st year | | 2 |
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| Master in Biomedicine, Professional Focus in Quality Assurance, 1st year | | 2 |
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| Master in Biomedicine, Professional Focus in focus in cosmetology, 1st year | | 2 |
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| Master in Biomedicine | | 2 |
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| Master in Biochemistry and Molecular and Cell Biology, Research Focus, 1st year | | 3 |
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| Master in Biochemistry and Molecular and Cell Biology, Teaching Focus, 1st year | | 3 |
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| Master en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée en bio-industrie, 1st year | | 3 |
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| Master in Biochemistry and Molecular and Cell Biology, Professional Focus in
Industrial Biochemistry, 1st year | | 3 |
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| Master en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée en bioinformatique et modélisation, 1st year | | 3 |
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| Lecturer : | Christian Damblon |
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Language(s) of instruction :
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| French language |
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Course contents :
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| The course is a practical application of NMR techniques studied in the course on Structural Analysis (CHIMI0650).
After a lesson summarizing the theoretical principles and practicalities of different NMR experiments, students will be asked to record themselves their own experiences protein in solution interacting with ligands.
The proposed experiments are the following 1D 1H with water suppression, 2D HSQC (HN). |
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Learning outcomes of the course :
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| Objective 1: Acquiring the ability to record adequately all NMR experiments required for the analysis of biological macromolecule in water
Objective 2: To understand the spectral information obtained by applying a sequence of pulses.
Objective 3: to choose experiences tailored to their research. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Course on structural analysis: CHIM-0213
Course on Structural biological Chemistry CHIM-0650 |
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Planned learning activities and teaching methods :
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| Practical work on the NMR spectrometer.
Preparation of the biological samples. Analysis of the spectra with appropriate computer programs : TOPSPIN-CCPNMR |
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Mode of delivery (face-to-face ; distance-learning) :
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| The course schedule will be determined with the students according to the availabilities of each and the availability of the spectrometer. |
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Recommended or required readings :
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| course CHIM-0213 (structural analysis)
cours CHIM-0650 (structural biological chemistry ) |
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Assessment methods and criteria :
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| Written report on the analysis performed by the students. |
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Work placement(s) :
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Organizational remarks :
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Contacts :
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| C. Damblon (C.Damblon@ulg.ac.be) |
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| Items online : |
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| cours 1-4 |
| Introduction à la spectroscopie RMNPrincipe physiques |
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