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| CHIM0433-1 | Proteomics
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| Duration : | 20h Th, 10h Pr |
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| Number of credits : |
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| Lecturer : | Edwin De Pauw, Marianne Fillet, Pierre Leprince |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the second semester |
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Course contents :
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| A course on modern proteomics covering the following parts:
- Comparative protemic analysis
- Clinical proteomics
- Mass spectrometry |
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Learning outcomes of the course :
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| Proteomics, partim comparative proteomic analysis (Pierre Leprince):
- Genomics and proteomics, promises and reality
- Protein chemistry facts useful for proteomic research
- Classical electrophoretic analysis techniques
- 2D-DIGE: principles and methods
- Samples preparation and processing
- Electrophoretic separation and gel analysis
- Tools for gel image analysis : Decyder DIA, BVA
- Spot picking and tryptic digestion
- Results validation
- Technical bias and limitations
-Examples of comparative proteomic analysis of physiological and pathological responses
A 2h practical demonstration is taking place in the proteomics laboratory at the CNCM (B36)
Clinical proteomic (Marianne Fillet)
- Introduction
- Novel technologies used for clinical proteomic
- Sample preparation for body fluid samples
- Statistical and bioinformatics tools
- Identification and validation of new diseases biomarkers;
- Prediction responses to therapy and diseases follow-up
A 2h practical demonstration is taking place in the proteomics platform at GIGA+2 (B24)
Proteomics: Partim 3, Mass Spectrometry (Edwin De Pauw) :
- The grounds of mass spectropmetry
- Ion sources: ESI, MALDI
- Mass spectrometers according to the needs
- Separation methods and coupling with ion sources (ESI, MALDI)
- Proteins identification (bottom up, top down)
- Relative and absolute quantification using isotopic labelling, label free quantitation
- Strctural methods on intact proteins: ECD, EDD, IRMPD, H/D exchange, ion mobility
- Non-covalent complexes
A two hours practical demonstration will be held in the mass spectrometry laboratory. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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Planned learning activities and teaching methods :
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| Three 2h demonstrations in the laboratories at CHU and GIGA |
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Mode of delivery (face-to-face ; distance-learning) :
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| Course is composed of three 6.5 h parts |
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Recommended or required readings :
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| available in pdf format on MyULg |
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Assessment methods and criteria :
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| written examination |
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Work placement(s) :
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Organizational remarks :
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Contacts :
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| Pierre Leprince
Centre for Cellular and Molecular Neurobiology (CNCM)
Université de Liège
CHU B36
B-4000 Liège
Belgique
Tél: 32 4 366 59 32
FAX: 32 4 366 59 12
pleprince@ulg.ac.be |
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