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| CHIB0006-1 | In vitro culture techniques (animal cells)
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| Duration : | 15h Th |
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| Number of credits : |
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| Lecturer : | Daniel Portetelle |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the first semester, review in January |
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Course contents :
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| Introduction: the use of animal cell culture
Biology of the cultured cells
The cell culture laboratory and its requirements
Laboratory safety and biohazards
The culture environment: substrate, gas phase, medium and temperature
Basic cell culture techniques
Quality control and biosafety of handling cells
Specialized techniques
Specific cell types and their requirements: a survey
Implications of animal cell culture in microbiology for growing of specific bacteria, virology for animal viruses, immunology and biotechnology; use in viability, cytotoxicity and modern toxicology as an alternative for animal experiment. |
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Learning outcomes of the course :
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| Overview of the basics of animal cell culture
Overview of their utilization and applications in microbiology, virology, biotechnology, immunology, modern toxicology and quality control
After completing the course the student is expected to
- know and control in vitro culture parameters
- use in vitro culture basic techniques. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Concepts of cellular and molecular biology of animal cells |
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Planned learning activities and teaching methods :
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Mode of delivery (face-to-face ; distance-learning) :
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| Lectures : 12h
Practical and personal works : 8h
Field-study trip and visits : 4h |
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Recommended or required readings :
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| Culture of animal cells (Freshney R.I.,ed.), 6°ed., 2010, Willey-Liss, New York, 576 pages
Text notes and Handbook
http://www.invitrogen.com/cellculturebasics ; PowerPoint available on line. |
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Assessment methods and criteria :
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| Written examination (80%)
Personal report (20%) |
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Work placement(s) :
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Organizational remarks :
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Contacts :
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| Portetelle, Daniel (Professeur ordinaire - Chef de service)
Microbiologie et Génomique
081 62 23 54
daniel.portetelle@ulg.ac.be |
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| Items online : |
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| Notes on line |
| PowerPoint on ecampus.
Electronic version of articles on MyULg. |
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