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| BIOL2024-3 | Applied molecular biology
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| Duration : | 36h Pr |
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| Number of credits : |
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| Lecturer : | Luc Willems |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the first semester, review in January |
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Course contents :
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| The course includes lectures describing the main molecular biology techniques that will be performed during practicals.
- Extraction and analysis of nucleic acids- real time PCR
- DNA cloning
- Cell transfection
- Reporter assays
- Western blot
- Confocal microscopy
- Flow cytometry
Another part of the course presents advanced techiques of molecular biology (e.g. microarrays, high throughput sequencing, transgenesis, interactomics).
The last part of the course includes workshops aiming at preparing an oral presentation in English of a research article and a project using different molecular biology techniques. |
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Learning outcomes of the course :
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| At the end of the course, the student should master the theoretical bases of the major molecular biology techniques. He should also be able to understand and perform a series of techniques in laboratory settings. He should also adequately interpret experimental results obtained during the practicals. Finally, he will be able to present a research paper in English and propose a project using molecular biology techniques. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| - BIOL2015-3 Molecular biology
- LANG2950-1 English level 1
- LANG2962-1 English level 2 |
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Planned learning activities and teaching methods :
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| The course will include:
- theoretical courses and seminars;
- practicals in the laboratory;
- teamwork to analyze research data and elaborate a project with the help of researchers (discussion workshops). |
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Mode of delivery (face-to-face ; distance-learning) :
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| Lectured
Laboratory training
Discussion workshops |
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Recommended or required readings :
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Assessment methods and criteria :
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| Oral presentation in English of:
- an analysis of molecular biology data (research article)
- a research project based on this article.
Response to questions pertaining to the presentation. |
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Work placement(s) :
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Organizational remarks :
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| Attendance at practical courses and workshops is mandatory. |
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Contacts :
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| Luc Willems
Research director of the FNRS
Professor at ULg
Biologie moléculaire - GxABT
13 avenue Marchal Juin
5030 Gembloux
Belgium
081-622157
luc.willems@ulg.ac.be |
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