 | | |
| BIOL0410-2 | Introduction to Morphological Methods
|
|
| Duration : | 10h Th, 5h Pr |
|
| Number of credits : |
|
|
| Lecturer : | Marie-Claire Gillet |
|
Language(s) of instruction :
|
| French language |
|
Organisation and examination :
|
| Teaching in the second semester |
|
Course contents :
|
| The morphological methods are essential in biomedical research. The key-elements for organelles, cells and tissues are studied.
Microscopes and basic principles are first described. Then, current technical preparations from biological samples will be explained, such as cuts, colourings, immunocytochemistry or immunohistochemistry.
The key-elements for observations will be developped, establishing relation between structure and function of organelles, cells and tissues. The principal quantitative methods, as well as the emergent methods of imagery which locate the molecules, peptides, lipids on sections (molecular and chemical signature) largely used in nanomedicine will be approached.
Plan:
1. Introduction
2. Microscopes and basic principles
3. Preparation of cells and tissues
4. Organelle, cell and tissue observation
5. Quantification and emergent techniques |
|
Learning outcomes of the course :
|
| This course is an introduction to the morphological methods used into the other courses, in particular the histology and in biomedical research.
The objectives are the training of the traditional microscopic methods and the modern methods of in situ analysis of molecules. |
|
Prerequisites and co-requisites/ Recommended optional programme components :
|
| Cellular biology |
|
Planned learning activities and teaching methods :
|
| Notes of course (dia) and practical work, bank of images and videos, didactic panels
5 courses of 2 hours
1 of practice |
|
Mode of delivery (face-to-face ; distance-learning) :
|
| face-to-face |
|
Recommended or required readings :
|
| Presentations (Power point)
Question exemples
On-line addition of showslides and questions |
|
Assessment methods and criteria :
|
| Written examination:
4 questions about the course (one of reflexion on methodology)
A practical question |
|
Work placement(s) :
|
| Room: 3/34; Institute of chemistry, B6C
1.Optical microscopy techniques
1.1 Taking away, fixing, cryofixation
1.2 Colourings
1.3 Histochemistry,
immunohistochemistry, ICC
1.4 Fluorescence
2. Colourings and cuts: practical application and discovery of a research laboratory
2.1 Smear of cells and Giemsa staining
2.2 Histological sections |
|
Organizational remarks :
|
| Laboratory notebook and tablier |
|
Contacts :
|
| Dr. Marie-Claire Gillet
Histologie-Cytologie
Mammalian Cell Culture Laboratory-GIGA R
B6c, Université de Liège
tél: +3243663403
Marie-claire.depauw@ulg.ac.be |
|
