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| BIOL0114-3 | Electronic microscopies
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| Duration : | 45h Th, 15h Pr |
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| Number of credits : |
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| Lecturer : | Philippe Compère |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the second semester |
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Course contents :
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| The course describes the physical principles and functioning of the major instruments in modern electron microscopy. It includes the following chapters :
1) Introduction and history of electron microscopy
2) Electron-material interactions (scattering and resulting particulate and electromagnetic radiations)
3) Instrument parts (vacuum pumps, electron guns and detectors, electromagnetic lenses)
4) Instrument operation and imaging in :
- scanning electron microscopy (SEM in high vacuum, low vacuum, controlled pressure conditions including "environmental" or "wet" modes
- transmission electron microscopy (TEM: different image, diffraction and high resolution modes)
- scanning transmission electron microscopy (STEM: principles, advantages and disadvantages)
5) Analytical electron microscopy
- X-ray microanalysis (EDS)
- Electron energy-loss spectroscopy(EELS) and energy filtering (EFTEM)
The course includes a practical learning on the preparation of samples for SEM/TEM as well as training sessions on the equipments of the Center of Applied Technology in Microscopy (CATµ-ULg).
This part constitutes an evaluated personnal work on a defined subjet, preferentially dedicaced to an original scientific question. |
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Learning outcomes of the course :
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| Functioning principles and imaging modes in electron microscopy.
Interpretation of the images.
Influences of the imaging mode and of tunning different parameter.
Learming preparation methods for SEM/TEM in biological or material sciences accoding to the orientation of the students.
Training as operator on SEM/TEM
Handle a short research using SEM/TEM with a good understanding of the obtained data. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Bases in Physics
Master degree students in Applied Sciences and Sciences
PhD students in Applied Sciences and Sciences including the Life Sciences, Geosciences, Agronomy, Archéology,...) |
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Planned learning activities and teaching methods :
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| Lectures |
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Mode of delivery (face-to-face ; distance-learning) :
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| Face-to-face |
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Recommended or required readings :
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Assessment methods and criteria :
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| Oral examination |
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Work placement(s) :
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Organizational remarks :
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| Lectures during the 2nd period |
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Contacts :
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| Compère Philippe
+32 4 366 50 63
pcompere@ulg.ac.be |
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