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| BIOC0714-1 | Production of recombinant proteins in eukaryotic systems
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| Duration : | 15h Th |
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| Number of credits : |
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| Lecturer : | Jacques Dommes |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the second semester |
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Course contents :
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| The main recombinant protein expression systems in eukaryotes are presented. The different systems are compared. The course also introduces criteria for choosing the most adequate system to meet specific scientific or industrial requirements. After an introductory part on bacterial systems, the course is organised into three parts dealing respectively with fungal, animal and plant systems. Here are the chapter titles:
1. Introduction: production of recombinant proteins in bacteria
2. Production of recombinant proteins in fungal systems
- Yeasts: the basics
- Saccharomyces cerevisiae
- Kluyveromyces lactis
- Pichia pastoris et Hanseluna polymorpha
- Yarrowia lipolytica
- Filamentous fungi
3. Production of recombinant proteins in animal cells
- Insect cells
- Mammalian cells
4. Production of recombinant proteins in plant systems
- Protein farming
- Unicellular green algae |
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Learning outcomes of the course :
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| The outcomes of this course are:
- to reveal the diversity of recombinant protein expression systems in eukaryotes
- to provide informations about strengths and weaknesses of the different systems
- to provide clues about the main criteria to be considered to choose the appropriate system, either for research use (small scale), or for the industrial production of a protein.
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Advanced knowledge of molecular biology and genetics, as well as of protein chemistry is requested. This course is suitable for students in the second year of the master in Biochemistry, Molecular and Cellular Biology, of the master in Bio-informatics and Modelisation, et of other masters in life sciences. It is also open to PhD students in the field of biochemistry and molecular and cellular biology. |
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Planned learning activities and teaching methods :
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| Most of the course is given face-to-face. It is illustrated with many examples. It also involves discussions on specific topics depending on student's interests. PowerPoint presentations are used throughout the course. |
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Mode of delivery (face-to-face ; distance-learning) :
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| Face-to-face (4 to 5 lessons, 3 hours each). |
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Recommended or required readings :
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| PowerPoint presentations, as well as a syllabus are available on eCampus. |
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Assessment methods and criteria :
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| QCM to evaluate acquired knowledge, completed by a few questions of problem solving. |
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Work placement(s) :
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Organizational remarks :
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Contacts :
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| Prof. J. Dommes (j.dommes@ulg.ac.be) |
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| Items online : |
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| eCampus |
| Materials available on eCampus |
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