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| SDOC0048-1 | Protein purification
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| Duration : | 15h Th |
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| Number of credits : |
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| Lecturer : | Moreno Galleni, Bernard Joris, André Matagne |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the second semester |
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Course contents :
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| I. Strategy (part 1); II. Theory of chromatography; III. Extraction, precipitation, concentration, dialysis ; IV. Size exclusion chromatography; V. Hydrophobic Interaction Chromatography; VI. Ion exchange; VII. Affinity chromatography; VIII. Other chromatographic methods; IX. Analytical methods for protein analysis (incl. mass spectrometry); X. Strategy (part 2); XI. Scale-up |
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Learning outcomes of the course :
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| The aim of these lectures is to teach students fundamentals in protein purification methods. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| The language used for the lectures will be english |
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Planned learning activities and teaching methods :
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| Lectures, given with the help of powerpoint presentations, blackboard and white chalk. Furthermore, a laboratory visit will be organized to present AKTA systems. |
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Mode of delivery (face-to-face ; distance-learning) :
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Recommended or required readings :
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| Scopes, R.K., Protein purification. Principles and Practice, 3rd Edition, Springer-Verlag, 1994 |
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Assessment methods and criteria :
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| irrelevant |
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Work placement(s) :
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Organizational remarks :
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| Students should register with Fabienne Julémont (fjulemont@ulg.ac.be) as soon as possible (program is generally available in May early). |
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Contacts :
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| André Matagne or Moreno Galleni, Centre d'Ingénierie des Protéines, Département des Sciences de la Vie, Institut de Chimie B6, Allée de la Chimie, 3, Université de Liège, B4000 Liège (Sart-Tilman) |
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