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| GENE0444-1 | Genetic engineering of pluricellular eukaryotes
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| Duration : | 15h Th |
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| Number of credits : |
| Master in Biochemistry and Molecular and Cell Biology, Research Focus, 1st year | | 2 |
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| Master in Biochemistry and Molecular and Cell Biology, Teaching Focus, 1st year | | 2 |
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| Master en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée en bio-industrie, 1st year | | 2 |
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| Master in Biochemistry and Molecular and Cell Biology, Professional Focus in
Industrial Biochemistry, 1st year | | 2 |
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| Master en biochimie et biologie moléculaire et cellulaire, à finalité spécialisée en bioinformatique et modélisation, 1st year | | 2 |
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| Lecturer : | Jacques Dommes |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the second semester |
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Course contents :
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| 1. Plant genetic engineering
- Transformation via Agrobacterium tumefasciens and the Ti plasmid
- Direct transformation methods
- Phytovirus vectors
- Inverse genetics in plants
- Metabolic engineering
- « Protein pharming »
- Production of recombinant protein in unicellular green algae
2. Production of recombinant proteins in insect cells
- Promoters
- Vectors
- Expression of recombinant proteins in silkworms
3. Cloning in mammalian cells
- Selection markers
- Transfection methods
- SV40 vectors
- Vaccinia vectors
- Alphavirus vectors
- Gene therapy
- Adenovirus vectors
- Herpes vectors
4. Transgenic animals
- Transfection methods
- Usefulness of transgenic animals
- « Dolly » : Multiplication of one individual through transfer of a somatic nucleus in an enucleated ovul
5. Regulatory aspects of experimenting or placing on the market of GMO |
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Learning outcomes of the course :
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| To get informed of the potential of genetic engineering in the study of living organisms, as well as in the improvement of species for human use and for the production of recombinant proteins. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Molecular biology and genetics (courses of the bachelier in Biology).
Genetic engineering (course of the BBMC and BIM master programs). |
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Planned learning activities and teaching methods :
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| Courses illustrated with Powerpoint slides. |
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Mode of delivery (face-to-face ; distance-learning) :
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| Lectures during second period. |
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Recommended or required readings :
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| Powerpoint presentations and lecture texts (partial coverage of the course) available through the eCampus system . |
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Assessment methods and criteria :
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| Oral exam (first exam session, as well as second one). |
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Work placement(s) :
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Organizational remarks :
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Contacts :
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| - Prof. J. Dommes (j.dommes@ulg.ac.be) |
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| Items online : |
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| Materials available on eCampus |
| - Powerpoint slides
- Syllabus
- Discussion forums |
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