University of Liege | Version française
Study programmes 2012-2013Last update : 18/06/2013
BOTA0401-1  Molecular approaches to the diversity of marine microorganisms

Duration :  15h Th, 15h Pr
Number of credits :  
Master in oceanography, Research focus, 2nd year2
Lecturer :  Annick Wilmotte
Language(s) of instruction :  
French language
Organisation and examination :  
Teaching in the second semester
Course contents :  
About 20 years ago, it became apparent that the study of the diversity of marine microorganisms cannot be conducted without a molecular approach, given that only a fraction of them can be isolated and cultured. In addition, as for the microorganisms that can be observed through a microscope, genetic diversity is larger than that which is determined on the basis of phenotypical characteristics (morphology....). The determination of diversity at the genetic level also allows us to understand ecological characteristics (geographical distribution, endemism, etc.).
Learning outcomes of the course :  
To give to the students the knowledge that will allow them, on one hand, to understand and read in a critical manner the literature concerning the diversity of marine microorganisms, and on the other hand, to use molecular techniques in order to solve problems concerning the molecular diversity and ecology on which they may be working (master thesis, PhD, etc).
Prerequisites and co-requisites/ Recommended optional programme components :  
Having basic knowledge of genetic material: DNA, RNA, proteins. If this prerequisite is not met, part of the course may be devoted to a review.
Planned learning activities and teaching methods :  
Practical work in a laboratory on a small problem involving diversity of marine microorganisms (often cyanobacteria) or oral presentation of a paper.
Mode of delivery (face-to-face ; distance-learning) :  
Face-to-face teaching, in 2nd semester 1) Definitions 2) Why? 3) Molecular markers of diversity 4) Molecular techniques 5) Phylogenetic analyses 6) How the genetic diversity is generated? 7) Examples of applications in molecular microbial ecology 8) Presentation of an article by students
Recommended or required readings :  
Copies of transparencies used in class
Assessment methods and criteria :  
1) Presentation of an article or report of laboratory work (if the student carries out a study in molecular diversity) 2) Exam on the theory
Work placement(s) :  
Organizational remarks :  
Contacts :  
A. Wilmotte, CIP, Chimie B6,
Phone : 04/366.38.56,
e-mail : awilmotte@ulg.ac.be


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