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| BIOL2024-3 | Applied molecular biology
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| Duration : | 24h Th, 12h Pr |
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| Number of credits : |
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| Lecturer : | Franck Dequiedt |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the first semester, examination in June |
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Course contents :
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| I-Overview1.Overview
2.Structure and properties of nucleic acids
II. Tools of Molecular Biology
1. Enzymes
2. Vectors
3. PCR
III. Molecular Biology Technics
1. Extraction and Purification of nucleic acids
2. Analysis of nucleic acids
a) Dosage
b) Restriction digest
c) Electrophoresis
d) Sequencing
e) Real-time PCR
f) PCR in molecular analysis
3. Cloning and gene transfert
a)Transgenesis
b)Functional genomics
c)Production of recombinant proteins
4. Two-hybrid
5. Emerging technics
-Transcriptomics
-Bio-informatics
-High throughput
Practical course
Session 1: Manipulation of Nucleic acids
RNA extraction form mammalian
RNA dosage by spectrophotometry
cDNA synthesis from purified RNA
Session 2: Cloning
Digest the PCR amplicon
Preparation of the cloning vector
Purification of digest products
Ligation and transformation into bacteria
Session 3 : Analysis of the cloning results and production of recombinant proteins
Analysis of cloning agar plates
DNA extraction from transformants |
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Learning outcomes of the course :
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| At the end of the course, the student must know the theoretical basis related to the most common techniques of molecular biology applied to research in academia or industry.
At the end of the practical course, the student should be able to :
directly implement in the laboratory some of the techniques seen during the theoretical course such as : DNA amplification by PCR, DNA digest by restriction enzymes, cloning procedures, protein expression in heterologous systems.
interprete results from the laboratory experiments |
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Prerequisites and co-requisites/ Recommended optional programme components :
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- knowing of the nucleic acid structure,
- recognizing the simple molecules that compose nucleic acids. Classifying these molecules based on their properties and functions
- knowing of the gene transcription and translation mechanisms and their constitutive steps
- Knowing of the various steps of protein synthesis and modifications
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Planned learning activities and teaching methods :
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| The theoretical course will included numerous examples and problems to prepare the students to the final evaluation |
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Mode of delivery (face-to-face ; distance-learning) :
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| Lectured
Laboratory training (3 sessions) |
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Recommended or required readings :
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Assessment methods and criteria :
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| Writing test : problems involving notions and know-how acquired during theoretical courses
The practical course (laboratory trainning) will be evaluated through a final report by the student |
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Work placement(s) :
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Organizational remarks :
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| Practical courses are mandatory |
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Contacts :
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| DEQUIEDT Franck, PhD
Associate Investigator of the FNRS
Associate Professor
University of Liège
GIGA-R
Laboratory of Protein Signaling
And Interactions (PSI)
Avenue de l'Hôpital, 1 (B34)
4000, Sart-Tilman
Belgium
tel: +32 4 366 24 26
fax: +32-4 366 45 34
fdequiedt@ulg.ac.be |
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