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| BIOL0410-2 | Introduction to Morphological Methods
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| Duration : | 10h Th, 5h Pr |
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| Number of credits : |
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| Lecturer : | Marie-Claire Gillet |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the second semester |
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Course contents :
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| The morphological methods are essential in biomedical research. The cells, tissues or organs are studied.
Traditional methods of optical microscopy, such the "live cell imaging", cuts and colourings will be described, as well as transmission, scanning electron microscopies and environmental microscope.
The quantitative methods, like those of morphometry, analysis of images will be presented.
Modern morphological methods such as AFM (microscopy with atomic force), digital holographic microscopy, as well as the emergent methods of imagery which locate the molecules, peptides, lipids on sections (molecular and chemical signature) largely used in nanomedicine will be approached.
Plan:
1. Cell and tissue observation
Historical background
Live versus fixed, in vitro versus in vivo
Example: bone tissue
2. Optical microscopy
2.1. Fixation
2.2. Coloration
3. Electron microscopy
3.1. Transmission electron microscope
3.2. Scanning electron microscope
3.3. Freezing / Etching
4. Nano-microscopy
5. In situ Visualisation of chemical agents
5.1. Live cell imaging
5.2. In vitro to in vivo
5.3. Molecular and chemical signature
6. Quantification |
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Learning outcomes of the course :
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| This course is an introduction to the morphological methods used into the other courses, in particular the histology and in biomedical research.
The objectives are the training of the traditional microscopic methods and the modern methods of in situ analysis of molecules. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Cellular biology |
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Planned learning activities and teaching methods :
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| Notes of course (dia) and practical work, bank of images and videos, didactic panels
5 courses of 2 hours
1 of practice |
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Mode of delivery (face-to-face ; distance-learning) :
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| face-to-face |
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Recommended or required readings :
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| Notes of course in preparation |
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Assessment methods and criteria :
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| Written examination:
Three questions about the course (one of reflexion on methodology)
A practical question |
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Work placement(s) :
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| Room: 3/34; Institute of chemistry, B6C
1.Optical microscopy techniques
1.1 Taking away, fixing, cryofixation
1.2 Colourings
1.3 Histochemistry,
immunohistochemistry, ICC
1.4 Fluorescence
2. Colourings and cuts: practical application and discovery of a research laboratory
2.1 Smear of cells and Giemsa staining
2.2 Histological sections |
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Organizational remarks :
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| Laboratory notebook and tablier |
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Contacts :
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| Dr. Marie-Claire Gillet
Histologie-Cytologie
Mammalian Cell Culture Laboratory-GIGA
B6c, Université de Liège
tél: +3243663403
Marie-claire.depauw@ulg.ac.be |
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