 | | |
| SDOC0048-1 | Purification des protéines
|
|
| Duration : | 15h Th |
|
| Number of credits : |
|
|
| Lecturer : | Moreno Galleni, Bernard Joris, André Matagne |
|
Language(s) of instruction :
|
| French language |
|
Course contents :
|
| I. Strategy (part 1); II. Theory of chromatography; III. Extraction, precipitation, concentration, dialysis ; IV. Size exclusion chromatography; V. Hydrophobic Interaction Chromatography; VI. Ion exchange; VII. Affinity chromatography; VIII. Other chromatographic methods; IX. Analytical methods for protein analysis (incl. mass spectrometry); X. Strategy (part 2); XI. Scale-up |
|
Learning outcomes of the course :
|
| The aim of these lectures is to teach students fundamentals in protein purification methods. |
|
Prerequisites and co-requisites/ Recommended optional programme components :
|
| The language used for the lectures will be english |
|
Planned learning activities and teaching methods :
|
| Lectures, given with the help of powerpoint presentations, blackboard and white chalk. Furthermore, a laboratory visit will be organized to present AKTA systems. |
|
Recommended or required readings :
|
| Scopes, R.K., Protein purification. Principles and Practice, 3rd Edition, Springer-Verlag, 1994 |
|
Assessment methods and criteria :
|
| irrelevant |
|
Organizational remarks :
|
| Students should register with Fabienne Julémont (fjulemont@ulg.ac.be) as soon as possible (program is generally available in May early). |
|
Contacts :
|
| André Matagne or Moreno Galleni, Centre d'Ingénierie des Protéines, Département des Sciences de la Vie, Institut de Chimie B6, Allée de la Chimie, 3, Université de Liège, B4000 Liège (Sart-Tilman) |
|
