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| GENE0210-1 | Genetics and molecular biology
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| Duration : | 60h Th, 30h Pr |
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| Number of credits : |
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| Lecturer : | N... |
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| Substitute(s) : | Bernard Peers, Marianne Voz, Jacques Dommes |
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Language(s) of instruction :
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| French language |
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Course contents :
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| 1. Genome structure, chromatin and nucleosomes (M. Voz)
2. DNA replication (B. Peers)
3. Alterations, reparations and mutations of DNA (B. Peers)
4. Homologous recombination (M. Voz)
5. Site-specific recombination and transposition (M. Voz)
6. Transcription (J. Dommes)
7. RNA splicing (J. Dommes)
8. Translation (J. Dommes)
9. The genetic code (J. Dommes)
10. Transcriptional regulation in Procaryotes (B. Peers and M. Voz)
11. Transcriptional regulation in Eukaryotes (J. Dommes)
12. Regulatory RNA (J. Dommes)
13. Gene regulation during development and evolution (B. Peers)
14. Genome analysis (M. Voz)
15. Techniques in molecular biology (M. Voz)
16. Protein - nucleic acid interactions (M. Voz)
17. Model organisms (B. Peers) |
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Learning outcomes of the course :
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| The first scope this course is to introduce the theoretical and practical bases of molecular genetics and molecular biology. Another aim is to give students all the background needed for the comprehension of many courses in the master in ecology and biology of the organism and in the master in biochemistry, molecular and cellular biology. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Introduction to genetic (BAC2 in biological sciences) |
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Planned learning activities and teaching methods :
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| The aim of the practical courses is to perform fingerprint by Southern bloting. Students will thus learn several molecular biology techniques from the isolation of DNA from their own blood to the Southern blot.
During the practical courses, several theoretical point will be reviewed: use of fingerprint and other techniques (PCR), notions of genetic variability between individuals (satellites séquences, polymorphism...), probe labeling and Southern blot techniques ... |
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Mode of delivery (face-to-face ; distance-learning) :
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| Lectures of 90 minutes, with PowerPoint presentations. During the first period, the lectures are programmed on Tuesdays and Wednesdays, from 8:30 to 10:00 am. During the second period, lectures will be given on Thursdays from 8:45 to 10:15 (weeks 1 to 10) and on Fridays (weeks 1 to 6). |
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Recommended or required readings :
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| - Molecular Biology of the Gene, VIth edition, J.D. Watson, T.A. Baker, S.P. Bell, A. Gann, M. Levine, R. Losick, Pearson, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, ISBN 0-321-50781-9
- Powerpoint presentations can be downloaded from MyULg (eCampus). |
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Assessment methods and criteria :
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| Report on practical courses (20% of the final score).
Oral examination on theory (80% of the final score). |
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Contacts :
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| - M. Voz, PhD, e-mail mvoz@ulg.ac.be
- B. Peers, PhD, e-mail bpeers@ulg.ac.be |
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| Items online : |
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| Available through internet |
| On eCampus |
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