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| LABO0432-2 | Techniques for cells and tissue cultures
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| Duration : | 30h Th |
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| Credits/ECTS : |
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| Holder(s) : | Marie‑Claire Gillet |
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| Language : | French language |
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| Course contents : | |
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| Course objective : | The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells in vitro
The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practices;
- handling, which constitutes the best training for the student. |
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| Prerequisites : | Good knowledge in cellular and tissue biology |
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| Workshops : | - Preparation of sterilized material and media
- Starting cells culture
- Congelation -decongelation
- Cells count
- Passaging
- Viability tests
- Observations (phase contrast and fluorescence microscopies)
- Mycoplasma detectionLocal: 3/62 - Institute of chemistry (B6c) |
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| Organization : | First semester
TP: Monday 8h30- 12h30
from the week 4
4 october 2010 until 13 december included
Course:
16 september, 8h15-10h15 (Sternon)
20 september, 10h30-12h30 (Sternon)
23 september, 18h30-10h30(Sternon)
NB: to be modified in function of the number of students and the teaching duties |
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| Written notes : | Syllabus: Culture de cellules - Les éditions de l'Université de Liège
Support DVD for workshop: La culture cellulaire
Notes de travaux pratiques
DIAs ( power point presentation) |
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| Assessment : | Practical :
- Laboratory book (/15) - 15 december
- Workshop (/15) - december
- Exercices and questions (/15): written examination in january
Theory: written examination in january (/55)
To be modifies in function of the teaching duties |
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| Contacts : | Dr M.C. De Pauw-Gillet
Institute of chemistry (B6c)
Allée de la chimie 3
Sart - Tilman
B 4000 Liège
Tél: 04/366.34.03
E-mail: Marie-Claire.DePauw@ulg.ac.be |
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| Remarks : | Blouse of laboratory obligatory
Laboratory book |
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