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| CHIM9258-1 | Thermodynamic and spectroscopic characterization of biomolecules
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| Duration : | 15h Th |
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| Credits/ECTS : |
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| Holder(s) : | Valérie Gabelica |
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| Language : | French language |
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| Course contents : | This course introduces various characterization methods applied to biomolecules (mainly nucleic acids and proteins):
1- Spectroscopy methods: UV-vis, infrared, circular dichroism, fluorescence, surface plasmon resonance,...
2- Calorimetry methods: isothermal titration calorimetry and differential scanning calorimetry.
3- Introduction to mass spectrometry of biopolymers.
In addition to the principles of each method, we will also discuss the data treatment necessary to extract thermodynamic or kinetic parameters relative to biomolecule folding or interactions. Finally, we will discuss the interpretation of these macroscopic parameters on the molecular
point of view (structure and dynamics). |
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| Course objective : | Understand which techniques is best appropriate to a given problem, what are the limitations of the different methods, what are the underlying assumptions in the data interpretation process. |
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| Organization : | 2nd semester, 7 or 8 lectures (2h) |
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| Written notes : | Copy of the slides. List of key review/tutorial articles. |
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| Assessment : | Written preparation, oral defense. |
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| Contacts : | Dr. Valérie Gabelica
Institut de Chimie, Bat B6c, Local R65/A
Tel. +32-4-366.3432
e-mail. v.gabelica@ulg.ac.be |
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