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| BIOC9233-1 | Structure and Dynamic of biological macromolecules
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| Duration : | 20h Th, 20h Pr, 20h Mon. WS |
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| Credits/ECTS : |
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| Holder(s) : | Paulette Charlier, André Matagne |
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| Language : | French language |
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| Course contents : | Partim Structure. The different experimental steps that will lead to the image of a biomolecule using the technique of X-ray diffractionby monocrystal will be described. Three examples illustrating the relations structure-function will be approached, on the basis of research articles describing structural analysis of different proteins illustrating the following 3 families: - proteins controlling the DNA transcription, - serine proteases, - membrane proteins.
Partim Dynamic. This series of lectures is based on the use of biophysical methods (mainly optical spectroscopies, presented into details for course CHIM9218-1 : Biospectroscopies et résonance magnétique nucléaire appliquée à la biologie) to study protein folding. The optical properties (i.e. absorbance, fluorescence, circular dichroism and infrared) of proteins will first be reviewed, followed by the analysis of concrete examples (e.g. bêta-lactamases, single-domain antibody fragments, lysozyme) found in the literature. |
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| Course objective : | Partim Structure. This course gives to the future biochemists the bases necessary to understand the principles of determination of 3D structures by X-ray crystallography in order to enable them to make an objective use of the crystallographic models.
Partim Dynamic. Give insights into the use of biophysical methods to study protein folding. |
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| Prerequisites : | Partim Structure. Course of physics of 1st cycle. Basic knowledge in X-ray crystallography and knowledge of the basic principles of the structures of proteins.
Partim Dynamic. BIOC0209-3 Chimie des macromolécules biologiques; CHIM9218-1 Biospectroscopies et résonance magnétique nucléaire appliquée à la biologie (partie biospectroscopie). |
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| Workshops : | Partim structure and dynamic. This lecture will be given in a classical form, including the use of a PowerPoint presentation, a chalk and a black board. |
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| Written notes : | Partim Structure. CD rom including Lectures powerpoint presentations, research articles, PDB files and tutorials will be available.
Reference books :
- "Crystallography made hook clear: With guide for users for macromolecular models." Scale Rhodos, Academic Press 2000
- "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999 CD roms:
- PS ² and Kinemage Supplement to "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999
Partim Dynamic. Lectures notes will be available |
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| Assessment : | A written or oral exam will be organized for each part of the course and it will be compulsory to obtain at least 8/20 for each of them. |
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| Contacts : | Partim Structure. Paulette CHARLIER
Institut de Physique, Bât. B5a - 1/41
Sart-Tilman, 4000 Liège
Call 00 32 (0)4/366.36.19
Fax 00 32 (0)4/366.37.48
E-mail : Paulette.Charlier@ulg.ac.be
Partim Dynamic. André Matagne, PhD, Professor, Enzymology and Protein Folding, Centre for Protein Engineering, Life Science Department, Institut de Chimie B6c (room 3/1), Allée de la Chimie, 3, University of Liège, B4000 Liège (Sart-Tilman), Tel.: +32 (0)4 3663419, Fax: +32 (0)4 3663396, Email: amatagne@ulg.ac.be, http://www.cip.ulg.ac.be/amatagne.htm (http://www.cip.ulg.ac.be/amatagne.htm" target=_blank eudora="autourl) |
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