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| BIOC0714-1 | Production of recombinant proteins in eukaryotic systems
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| Duration : | 15h Th |
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| Credits/ECTS : |
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| Holder(s) : | Jacques Dommes |
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| Language : | French language |
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| Course contents : | 1. Introduction : production of recombinant proteins in bacteria
2. Yeast systems
- Basic concepts
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Saccharomyces cerevisiae
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Kluyveromyces lactis
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Pichia pastoris
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Hanseluna polymorpha
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Yarrowia lipolytica
- Filamentous fungi
3. Animal cell systems
- Insect cells
- Mammalian cells
4. Protein farming
- Genetically modified plants
- Genetically modified algi
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| Course objective : | - Overview of the different available systems for the production of recombinant proteins in eukaryotic organisms.
- Understanding of the strengths and weaknesses of each system, so as to allow to select a system adapted to a specific protein and to a specific application. |
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| Prerequisites : | Genetics and molecular biology (Biology BAC3 level). |
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| Workshops : | No practicals. |
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| Organization : | Lectures organised in half-day sessions between February and April. Time table to be discussed with the different trainees. |
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| Written notes : | PowerPoint presentations. |
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| Assessment : | Written exam (multiple choice questions). |
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| Contacts : | Prof. Jacques DOMMES
j.dommes@ulg.ac.be |
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