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| LABO0432-1 | Techniques for cells and tissue cultures
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| Duration : | 5h Th, 20h Pr |
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| Credits/ECTS : |
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| Holder(s) : | Marie‑Claire Gillet |
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| Language : | Langue française |
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| Course contents : | The course is subdivided in the following way:
- cell lines
- aseptic techniques
- culture environment
- primary cultures
- optical and electron microscopies techniques
- cloning and cell type selection
- caracterization
- transformed phenotype
- contaminations
- instability, variation and conservation
- quantification and experimental pojects
- viability and cytotoxicity measurement
- tridimensional culture. |
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| Course objective : | The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells in vitro
The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practices;
- handling, which constitutes the best training for the student. |
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| Prerequisites : | Good knowledge in cellular and tissue biology |
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| Workshops : | - Preparation of sterilized material and media
- Starting cells culture
- Congelation -decongelation
- Cells count
- Passaging
- Viability tests
- Observations (phase contrast microscopy)
- Mycoplasma detection
Local: 3/62 - Institute of chemistry (B6c) |
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| Organization : | First semester
TP: Monday 8h30- 12h30
from the third week
28 september 2009 until 15 december included
Course:
15 september, 15h30-17h30
16 september, 8h45-10h15
21 september, 10h30-12h30 |
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| Written notes : | Syllabus: Culture de cellules - Les éditions de l'Université de Liège
Support DVD: La culture cellulaire
Notes de travaux pratiques
DIAs ( power point presentation) |
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| Assessment : | Practical :
- Laboratory book (/10) - 15 december
- Oral presentation (/10) - 7 and 14 december
- Exercices and questions (/20): written examination in january
Theory: written examination in january (/60) |
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| Contacts : | Dr M.C. De Pauw-Gillet
Institute of chemistry (B6c)
Allée de la chimie 3
Sart - Tilman
B 4000 Liège
Tél: 04/366.34.03
E-mail: Marie-Claire.DePauw@ulg.ac.be |
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| Remarks : | Blouse of laboratory obligatory
Laboratory book
One photo |
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