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| MICR0711-1 | Microbiology - Partim 1 : Algology and mycology - Partim 2 : Bacteriology - Partim 3 : Virology
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| Duration : | Partim 1 : Algology and mycology : 20h Th, 10h Pr Partim 2 : Bacteriology : 20h Th, 10h Pr Partim 3 : Virology : 20h Th, 10h Pr
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| Credits/ECTS : |
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| Holder(s) : | Partim 1 : Algology and mycology : Vincent Demoulin
Partim 2 : Bacteriology : Bernard Joris
Partim 3 : Virology : Jacques Piette
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| Language : | Langue française |
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| Course contents : |
 |  | Partim 1 : Algology and mycology |

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 | A first lecture exposes biochemical and ultrastructural characters whose variation among great groups of algae anf fungi makes that under an often simple morphology those organisms display a much greater diversity of organisation than higher plants or animals. One lecture is devoted to blue-green algae, whose procaryotic structure places in a peculiar position ("cyanobacteria") but whose ecological rôle is similar to that of other algae. Main themes: importance in present and Precambrian ecosystems, nitrogen fixation, toxins. Four lectures are devoted to eucaryotic algae, mainly red, brown and green, as well as the main planctonic ones (dinoflagellates, diatoms...). Main themes: evolution of eucaryotes (origin, loss or gain of chloroplasts, origin of higher plants), ecological and economical (mucilages) importance of benthic algae, primary production and toxic blooms of planctonic ones, symbiosis of dinoflagellates... Four lectures are devoted to the introduction to the fungal way of life and the main group, the Ascomycetes. Among those a special attention is given to yeasts as technological and scientific tools (small fully sequenced genome, transformations, fermentations). Other themes: parasites and symbionts (lichens), mycotoxins and antibiotics. Two lectures are devoted to Basidiomycetes, insisting on their importance as mycorhizal symbionts or wood-rotters (ecological importance, destruction of timber, biotechnological potential of the capacity to metabolize lignin). One lecture is devoted to other fungi with emphasis on Mucorales (moulds) and Glomales (endomycorhizae), Oomycetes (economical and historical importance) and Dictyostelium (tool of molecular biology). Practical teaching is based on the collection of fungi in the surrounding of the Botanical Institute, their observation in the laboratory and attempts at cultivation. Algae from the pond near the Institute are treated in the same way.
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 |  | Partim 3 : Virology |

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 | Chapter I : Basis of virology Chapter II : The various infectious cycles Chapter III : The various steps of an infectious cycle Chapiter IV : The possible origin of latency in relationship with several pathologies Chapter V : How can we classify virus- the Baltimore classification. Chaptre VI : Class V : Orthomyxoviridae; paramyxoviridae; rhabdoviridae. Chapter VII : Class IV : Picornaviridae; togaviridae; flaviviridae; coronaviridae. Chapter VIII : Class III : Reoviridae. Chapter IX : Class I : Herpesvirus; papovaviridae; adénoviridae; poxviridae. Chapter X : Class II : Parvoviridae; bactériophages FX174 & M13 Chapter XI : Class VI : Retroviridae, lentivirus & HIV Chapter XII : Class VII : Hepdnaviridae; HBV Chapter XIII : The viruses that escape the immune system. |
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| Course objective : |
 |  | Partim 1 : Algology and mycology |

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 | The aim is to make students familiar with the major organisms of algal and fungal types which present an interest for general biology, either through their peculiarity in morphology, ultrastructure or biochemistry, or their ecological importance, their phylogenetic position or economical impact. By defining the way of life (ecological and physiological peculiarities) and evolutionary position of the studied organisms, emphasis is put on advantages and inconveniences they present as experimental or biotechnological tools. |
 |  | Partim 3 : Virology |

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 | Obtaining a general view of the diversity of the world of virus and of the complexicity of the interactions between host and virus. Helping in the general understanding on how the immune response to virus is triggered by the host. |
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| Prerequisites : |
 |  | Partim 1 : Algology and mycology |

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 | A minimal knowledge of general biology and biochemistry. |
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| Workshops : |
 |  | Partim 1 : Algology and mycology |

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 | Laboratory courses number four half-days (two for algae, two for fungi) in laboratories located at the -1 floor of the Botanical Institute B.22 in the Sart Tilman campus. Fresh material is collected in the surroundings fo the building, located near the forest, at the beginning of the course. Some of the laboratory manipulations will be common to the bacteriology course. |
 |  | Partim 3 : Virology |

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 | The pratical course is integrated with Immunology |
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| Organization : |
 |  | Partim 1 : Algology and mycology |

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 | Assitance to the lectures is important for they are illustrated by a large number of colour slides. For laboratory courses one needs A4 paper, pencil, eraser and a pocket lens 10-15x. |
 |  | Partim 3 : Virology |

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 | Course of 90 min |
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| Written notes : |
 |  | Partim 1 : Algology and mycology |

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 | Full notes ("Les champignons, notions de base" by G. Castillo and V. Demoulin and "Utillisation biotechnologique des champignons" by V. Demoulin) are available for fungi. Partial notes are distributed for algae. Various books are presented for information to students interested by further data, in the present or future, but they are not necessary to understand the course. |
 |  | Partim 3 : Virology |

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 | No written notes. However, the students will receive copies of the overheads shown during the course. Text book is Virology (Ed B. Fields et al, Lippincott-Ravel et al. , New-York, USA) and Understanding Viruses by Teri Shors (Ed. Jones & Bartlett, USA) |
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| Assessment : |
 |  | Partim 1 : Algology and mycology |

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 | Final note is made up for one fourth by appreciations of the laboratory reports and three quarters by the written examination (multiple choices form). As with the two other partims, 8/20 is necessary for succeeding in the whole course. |
 |  | Partim 3 : Virology |

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 | Oral examination. Two questions on the theoretical course |
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| Contacts : |
 |  | Partim 1 : Algology and mycology |

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 | Teacher: V. Demoulin, professeur ordinaire, Institut de Botanique, B.22 (P.70) in the Sart Tilman campus. Tel. 04/366.38.53, e-mail: V. Demoulin@ulg.ac.be If no answer on the phone, message possible after 8 ringings. Assistant: G. Castillo, logisticien, e-mail: G.Castillo@ulg.ac.be |
 |  | Partim 3 : Virology |

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 | Jacques Piette, Directeur de Recherches FNRS et Professeur Adjoint. Laboratoire de Virologie & Immunologie, Institut de Pathologie, Bât. B23, 4000 Liège 1 (Sart Tilman) Tél : 04/366.24.42, Fax : 04/366.24.33, E-mail : jpiette@ulg.ac.be |
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| Remarks : |
 |  | Partim 3 : Virology |

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 | It is important to notice that as for the two others parts of te course, an evaluation note of 8/20 is required in order to succeed. |
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