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| LABO0432-1 | Techniques for cells and tissue cultures
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| Duration : | 5h Th, 20h Pr |
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| Credits/ECTS : |
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| Holder(s) : | Marie‑Claire Gillet |
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| Language : | Langue française |
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| Course contents : | The course is subdivided in the following way:
- The cell in culture: environment, primary cell, lines, contaminations, cloning.
- Methods of separation, characterization
- Tests of cytotoxicity.
- Specialized techniques.
- Methods of tissue engineering. |
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| Course objective : | The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells, in vitro so that the latter are able to cultivate cells .
The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practice;
- handling, which constitutes the best training for the student. |
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| Prerequisites : | Good knowledge in cellular and tissue biology |
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| Workshops : | -Starting cells culture
-Congelation -decongelation
-Cells count
-Passaging
-Viability tests
-Observations (phase contrast microscopy)
Local: 3/62 - Institute of chemistry (B6c) |
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| Organization : | First semester
Monday 8h30- 12h30
from the 4 week
10 october 2005 until 28 november included |
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| Written notes : | Syllabus: Culture de cellules - Les éditions de l'Université de Liège |
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| Assessment : | Theoretical and practical questions - written examination |
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| Contacts : | Dr M.C. De Pauw-Gillet
Institute of chemistry (B6c)
Allée de la chimie 3
Sart - Tilman
B 4000 Liège
Tél: 04/366.34.03
E-mail: Marie-Claire.DePauw@ulg.ac.be |
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| Remarks : | Blouse of laboratory obligatory |
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