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| BIOC0001-1 | Genetic, molecular biology and chemistry of biological macromlecules
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| Duration : | 30h Th, 30h Pr |
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| Credits/ECTS : |
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| Holder(s) : | Jacques Dommes, Moreno Galleni |
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| Language : | Langue française |
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| Course contents : | 1. The main classes of RNA
2. Protein synthesis
3. Using the genetic code
4. Protein localisation requires specific signals
5. Transcription
6. Regulatory RNA
7. Promoters and enhancers
8. Regulating eukaryotic transcription
9. RNA splicing and processing
10. Interactions and bounds
11. Organisation of the polypeptide chain
12. Stabilisation and folding of the polypeptide chain
13. Structure-activity relationship
14. Deanturation, inactivation and stability of proteins
15. Determination of protein concentration
16. Analyse of the amino acid sequence of a polypeptide chain
17. Introduction to protein purification
18. Electrophoresis |
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| Course objective : | The first scope of the lectures is to introduce the theoretical and practical bases of the molecular gentic and biology. The second sope will be to improve the student knowledge in advanced biochemistry. The major aim of the course will be to help students for the comprehension of other lectures of the master in ecology and biology of the organism and for the master in biochemistry, molecular and cellular biology. |
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| Prerequisites : | Introduction to genetic (BAC2 in biological sciences)
Introduction to Biochemistry and thermodynamic of biological systems. (BAC2 in biologicals sciences) |
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| Workshops : | The pratical courses will be organised on Mondays and Thursdays during the weeks 8 to 11 of the second semester.
The pratical courses will be based on basic manupulations of molecular biology and protein chemistry. The students will learn the basic protocols for the cloning of a cDNA fragment into an expression plasmid. The plasmid will be transformed into a bacterial host. The protein will be expressed, purified and analysed. |
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| Organization : | Molecular genetics and molecular biology (Prof. J. Dommes) : Mondays and Wednesdays of weeks 8 - 14 of first semester.
Chemistry of macromolecules (Prof. M. Galleni) : Mondays and Thursdays of weeks 1 - 7 during second semester. |
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| Written notes : | Genetics and molecular biology :
- Essential Genes, B. Lewin, Pearson Education Inc., Upper Saddle River, USA, ISBN 0-13-201636-2.
- Molecular Biology of the Gene, VIth edition, J.D. Watson, T.A. Baker, S.P. Bell, A. Gann, M. Levine, R. Losick, Pearson, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, ISBN 0-321-50781-9
- Powerpoint presentations can be downloaded from MyULg (WebCT).
Partim chemistry of proteins
Power Point presentation + Syllabus |
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| Assessment : | Report written during the practical courses.
Written examination. |
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| Contacts : | - J. Dommes, Prof. Ordinaire, e-mail J.Dommes@ulg.ac.be 04/366.38.99
- M. Galleni, Chargé de cours, e-mail mgalleni@ulg.ac.be 04/366.35.49
- C. Gauthier, assistante, e-mail carole.gauthier@ulg.ac.be 04/366.38.73
- R. Berlémont, assistant, e-mail rberlemont@student.ulg.ac.be 04/366.33.32
- G. Feller, chef de travaux, e-mail g.feller@ulg.ac.be 04/366.33.43 |
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| Remarks : | References
- Essential Genes, B. Lewin, publié par Pearson Education Inc., Upper Saddle River, USA, ISBN 0-13-201636-2.
- Proteins: structures and molecular properties, T.E. Creighton, publié par WH Freeman and Company, New York, USA, ISBN 0-7167-7030-X |
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| Items online : |
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| WebCT |
| Online course on WebCT |
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