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| ZOOL0212-1 | Cellular and tissue ultrastructure
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| Duration : | 15h Th, 15h Pr |
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| Credits/ECTS : |
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| Holder(s) : | Philippe Compère, Marc Thiry |
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| Language : | Langue française |
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| Course contents : | Partim of M. Thiry
1. cytochemical methods
2. autoradiographic methods
3. immunocytological methods
4. Molecular biology based-procedures adapted to electron microscopy
5. in situ hybridization technique |
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| Course objective : | Partim of M. Thiry (7h30 + 7h30)
To give a sketch of the different approaches of visualization used in microscopy. The nucleus will be used as main model. |
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| Prerequisites : | Goods basics notions in biology, biochimy, cell biology, immunology and animal histology. |
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| Organization : | This teaching is given during the first quadrimester according to an horary that will be communicated in the beginning of term day. The pratical exercices, about the two partims (3 sessions) constitue to illustrate some notions that were saw in the teoretical course thanks to the use of the scanning and transmission electron microcopy, the student is bring along to use himself it. These exercices form the subject of an individuel evaluation that count for 50% of the final cote of the exam. |
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| Written notes : | Partim of M. Thiry
Documents are given in every lesson.
References works :
- Staining methods for sectioned material, P.R. Lewis & D.P. Knight, Practical methods in electron microscopy, NHPC (1977)
- Methods in ultrastructural cytochemistry of the nucleus, G. Moyne, Progress in histochem cytochem, 13 n°1, Gustav Fischer Verlag (1980)
- An introduction to immunocytochemistry: current techniques and problems, J.M. Polak & S. Van Noorden, microscopy handbooks,11, OUP (1984)
- Immunolabelling for electron microscopy, J.M. Polak & I.M. Varndell, Elsevier (1984)
- Hybridation in situ: méthodes pratiques, A. Calas, B. Bloch, J.-G. Fournier & A. Trembleau, SF.M.E., ( 1992)
- Fine structure immunocytochemistry, G. Griffiths, Springer Verlag (1993)
- Ultrastructural methods for nucleic acid detection by immunocytology, M. Thiry, Progress in histochem cytochem, 34 n°2, Urban & Fischer (1999) |
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| Assessment : | The two theoretical partims that constitue this course title form the subject only one oral exam with the presence of the two professors |
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| Contacts : | Thiry Marc
Laboratory of cell biology
Institut A. Swaen (Building L3) 20, rue de pitteurs, 4020 Liège or Center of Cell and molecular Neurobiology, CHU (Building B36), firth floor of pharmacy tower, Sart Tilman, 4000 Liège
Phone number : 32 4 3665166
E-mail : mthiry@ulg.ac.be |
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