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| CHIM0255-2 | Sructure and dynamics of biological macromolecules
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| Duration : | 50h Th, 30h Pr |
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| Credits/ECTS : |
| "licencié" biochemical sciences, 2nd year | | Deuxième quadrimestre | | 8 |
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| Master in Biochemistry, cell and molecule biology, in-depth approach, 1st year | | Toute l'année | | 8 |
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| Master in Biochemistry, cell and molecule biology, didactic approach, 1st year | | Toute l'année | | 8 |
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| Master in Biochemistry, cell and molecule biology, specialized approach, 1st year | | Toute l'année | | 8 |
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| Master in Biological Sciences | | Toute l'année | | 8 |
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| Holder(s) : | Paulette Charlier, Pierre Colson, Gabriel Llabres, André Matagne |
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| Language : | Langue française |
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| Course contents : | Structure part (20h+20h):
X-rays: A rapid overflight of the various necessary steps that will lead to the image of a biomolecule is carried out in the first 4 hours of theoretical course. Three examples illustrating the relations structure-function will be approached in the 6 last hours of theoretical course: - proteins controlling the DNA transcription, - serine proteases, - membrane proteins.
The 10 hours of TP will be dedicated to the analysis and the discussion of articles of structure concerning the 3 families of proteins described.
Dynamics part
The lecture begins with a short review of the spectral transitions. Afterwards, the following spectroscopic techniques are studied in detail : uv-visible absorption, linear and circular dichrosms, fluorescence and its polarisation, I.R. spectroscopy and surface plasmonic resonance. For each technqiue, experimental aspects and applications to the study of protein and nucleic acid conformations and of their interactions with small ligands will be developed |
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| Course objective : | Structure part (20h+20h):
X-rays: This course gives to the future biochemists the bases necessary to understand the principles of determination of 3D structure by X-ray crystallography in order to enable them to make an objective use of the crystallographic models.
Dynamics part
Increase the qualifications of the student concerning the spectroscopic methods available for the study of the conformational dynamics of proteins (folding and unfolding), nucleic acids and their interactions. |
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| Prerequisites : | Structure part : Course of physics of 1st cycle.
Xrays: Basic knowledge in X-ray crystallography (2° candidature in Biology or Chemistry) and knowledge of the basic principles of the structures of proteins (1st Licence in Biochemistry).
Dynamics part :
Chemistry of Biological Macromolecules Functional properties of biological macromolecules |
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| Organization : | Structure Part:
Xrays:
Theory: 4 hours dedicated to the determination of the biomolecules structures by X-ray crystallography - 6 hours dedicated to the illustration structure-function with 3 examples.
TP: Analysis and critical discussion of articles in relation to the examples of structures described.
Dynamics part :
This lecture will be given as a classical form (as interactive as possible) by the two teachers according to their specific qualifications |
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| Written notes : | Structure part:
Xrays: Lecture presentation will be available on CD rom.
Reference books :
- "Crystallography made hook clear: With guide for users for macromolecular models." Scale Rhodos, Academic Press 2000
- "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999 CD roms:
- PS ² and Kinemage Supplement to "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999
NMR: Notes of course available.
Dynamics part :
Lecture notes will be available.
Reference books :
- Biophysical Chemistry, Cantor and Schimmel
- Intrduction to protein chemistry, Branden and Tooze
- Principles of Physical Chemistry, Van Holde et al.
- Biological Spectroscopy, Campbell and Dwek
-Physical Biochemistry, Sheehan |
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| Assessment : | Le mode d'évaluation sera choisi en concertation avec les étudiants. La réussite du cours (i.e. comprenant toutes ses parties) ne sera possible qu'à la condition d'obtenir au moins 8/20 auprès de chaque enseignant |
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| Contacts : | Structure part:
Xray: Dr. Paulette CHARLIER
Institut de Physique, Bât. B5a - 1/41
Sart-Tilman, 4000 Liège
Call 00 32 (0)4/366.36.19
Fax 00 32 (0)4/366.37.48
E-mail : Paulette.Charlier@ulg.ac.be
NMR: Pr. Gabriel LLABRES
Call 00 32 (0)4/366.36.13
Fax 00 32 (0)4/366.28.13
E-mail : Gabriel.Llabres@ulg.ac.be
Dynamics part :
Pierre COLSON, Chargé de Cours
Office : 3/73B, Chemistry Institute (B6c), Sart-Tilman, 4000 - Liège 1 , Belgium
Call : 00 32 (0)4/3663406
Fax : 00 .2 (0)4/3664577
e-mail : P.Colson@ulg.ac.be
André MATAGNE, Chercheur Qualifié FNRS - Institut de Chimie, Sart-Tilman (B6c) - Bureau 3/1 Call : 00 32 (0)4/366.34.19 - Sec : 00 32 (0)4/366.33.41
Fax : 00 32 (0)4/366.33.64
e-mail : amatagne@ulg.ac.be |
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