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| BIOC0001-1 | Genetic, molecular biology and chemistry of biological macromlecules | |||||
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| Duration : | 30h Th, 30h Pr | |||||
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| Holder(s) : | Jacques Dommes, Moreno Galleni | |||||
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| Course contents : | 1. Introduction 8. Interactions and bounds 9. oragnisation of the polypeptide chain 10. Stabilisation and folding of the polypeptide chain 11. Structure-activity relationship 12. Deanturation, inactivation and stability of proteins 13. Determination of protein concentration 14. Analyse of the amino acid sequence of a polypeptide chain 15. Introduction to protein purification 16. Electrophoresis | |||||
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| Course objective : | The first scope of the lectures is to introduce the theoretical and practical bases of the molecular gentic and biology. The second sope will be to improve the student knowledge in advanced biochemistry. The major aim of the course will be to help students for the comprehension of other lectures of the master in ecology and biology of the organism and for the master in biochemistry, molecular and cellular biology. | |||||
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| Prerequisites : | Introduction to genetic (BAC2 in biological sciences) Introduction to Biochemistry and thermodynamic of biological systems. (BAc2 in biologicals sciences) | |||||
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| Workshops : | Time schedule. The pratical courses will be organised on two complet weeks (weeks 9 and 10) Program The pratical courses will be based on basic manupulation of molecular biology and protein chemistry. The students will learn the basic protocol for the cloning of a cDNA fragment into a bacterial expression plasmid. The plasmid will be transformed into a bacterial host. The protein will be expressed, purified and analysed. | |||||
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| Organization : | Second quater semester each monday and tuesday of weeks 1-13 8h45 -10h15: Galleni M 10h45- 12h15: Dommes J | |||||
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| Written notes : | Partim chemistry of proteins Power Point presentation + Syllabus | |||||
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| Assessment : | Report written during the parctical courses Oral examination | |||||
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| Contacts : | - J. Dommes, Prof. Ordinaire, e-mail J.Dommes@ulg.ac.be 04/366.38.99 - M. Galleni, Chargé de cours, e-mail mgalleni@ulg.ac.be 04/366.35.49 - C. Gauthier, assistante, e-mail carole.gauthier@ulg.ac.be 04/366.38.73 - R. Berlémont, assistant, e-mail rberlemont@student.ulg.ac.be 04/366.33.32 - G. Feller, chef de travaux, e-mail g.feller@ulg.ac.be 04/366.33.43 | |||||
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| Remarks : | References - Essential Genes, B. Lewin, publié par Pearson Education Inc., Upper Saddle River, USA, ISBN 0-13-201636-2. - Proteins: structures and molecular properties, T.E. Creighton, publié par WH Freeman and Company, New York, USA, ISBN 0-7167-7030-X | |||||
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ULg : Students and Studies Administration - Academic Affairs
Contact : Monique Marcourt, direction A.E.E.
Date of data : 18/05/2007
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