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| ZOOL0212-1 | Cellular and tissue ultrastructure | |||||
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| Duration : | 15h Th, 15h Pr | |||||
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| Credits/ECTS : |
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| Holder(s) : | Philippe Compère, Marc Thiry | |||||
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| Course contents : | Partim of M. Thiry
1. cytochemical methods 2. autoradiographic methods 3. immunocytological methods 4. Molecular biology based-procedures adapted to electron microscopy 5. in situ hybridization technique | |||||
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| Course objective : | Partim of M. Thiry (7h30 + 7h30)
To give a sketch of the differents visuals approachs using in microscopy. The nucleus will be used as main model. | |||||
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| Prerequisites : | Goods basics notions in biology, biochimy, cell biology, immunology and animal histology. | |||||
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| Organization : | This teaching is given in the courses of the first quadrimester pursuant an horary that will be communicated in the beginning of term day. The praticals exercices, about the two partims (3 sessions) constitue to illustrate some notions that were saw in the teoretical course thanks of the utilisation of the sweep and the transmission microcopy, the student is bring along to use himself it. This exercices form the subject of an individuel evaluation that count for 50% of the final cote of the exam. | |||||
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| Written notes : | Partim of M. Thiry
Documents are given in every lesson. References works : - Staining methods for sectioned material, P.R. Lewis & D.P. Knight, Practical methods in electron microscopy, NHPC (1977) - Methods in ultrastructural cytochemistry of the nucleus, G. Moyne, Progress in histochem cytochem, 13 n°1, Gustav Fischer Verlag (1980) - An introduction to immunocytochemistry: current techniques and problems, J.M. Polak & S. Van Noorden, microscopy handbooks,11, OUP (1984) - Immunolabelling for electron microscopy, J.M. Polak & I.M. Varndell, Elsevier (1984) - Hybridation in situ: méthodes pratiques, A. Calas, B. Bloch, J.-G. Fournier & A. Trembleau, SF.M.E., ( 1992) - Fine structure immunocytochemistry, G. Griffiths, Springer Verlag (1993) - Ultrastructural methods for nucleic acid detection by immunocytology, M. Thiry, Progress in histochem cytochem, 34 n°2, Urban & Fischer (1999) | |||||
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| Assessment : | The two theoreticals partims that constitue this course title form the subject only one oral exam with the presence of the two professors | |||||
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| Contacts : | Thiry Marc Laboratory of cell biology, Institut A. Swaen (Building L3) 20, rue de pitteurs, 4020 Liège Phone number : 32 4 3665160/69 E-mail : mthiry@ulg.ac.be | |||||
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ULg : Students and Studies Administration - Academic Affairs
Contact : Monique Marcourt, direction A.E.E.
Date of data : 27/02/2006
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