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| GENE0204-1 | Plant genetic engineering | |||||
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| Duration : | 30h Th, 30h Pr | |||||
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| Credits/ECTS : |
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| Holder(s) : | Jacques Dommes | |||||
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| Course contents : | Genetic engineering is a pool of techniques used to develop genetically modified organisms, i.e. organisms in the genome of which one or a few foreign genes are inserted. It allows the rapid development of improved plant varieties or plants producing vaccines or therapeutics. But it is also a valuable tool in fundamental research in plant physiology. The course contains 11 chapters. The 4 first ones present the different plant transformation methods. The fifth one deals with inverse genetics and gene silencing. Chapters 6 and 7 present examples of genetically modified food plants, and of plants producing recombinant proteins (protein farming). One chapter deals with the phenomenons of instability of transgene expression. The 3 last chapters compare genetic engineering approaches to traditional plant breeding, dealing with the risks for health and for the environment and also with the regulations concerning the confined use and the deliberate release of genetically modified organisms. | |||||
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| Course objective : |
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| Prerequisites : | This course is given in the fourth year. The required background is given :
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| Workshops : | Practicals will involve genetic transformation of a yeast and monitoring of the production of a recombinant protein by this genetically modified organism. Other aspects of the theoretical course will be illustrated. | |||||
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| Organization : | Lectures : the timetable will be available on the first day of the academic year. All lectures will be given in one of the lecture rooms of B-22 building. Practicals : half-day sessions in one of the student laboratories of the B-22 building. Each student should bring a white coat and a notebook. | |||||
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| Written notes : | Syllabus (containing most pictures used during lectures, about 200 p.) is avilable (Cercle de Biologie). PowerPoint presentations are also available (paper copy and CD-ROM). Facultative reference books : - Recombinant DNA: J.D. Watson, M. Gilman, J. Witkowski, M. Zoller, 1992. Scientific American Books, W.H. Freeman and Co., New York, USA. - Droit et Génie Génétique - Premier bilan international et européen des réglementations et des nouvelles politiques : S. Soumastre éditeur, 1994. Elsevier, Paris, France. - Recombinant proteins from plants - Production and isolation of clinically useful compounds : C. Cunningham et A.J.R. Porter, éditeurs, 1998. Humana Press, Totowa, New Jersey, USA. - Biochemistry & Molecular Biology of Plants : B.B. Buchanan, W. Gruissem, R.L. Jones, 2000. American Society of Plant Physiologists, Rockville, USA. Other interesting books : - Gènes VI : B. Lewin, 1997. De Boeck Université, Paris, France. | |||||
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| Assessment : | They will be an oral examination during which the student will have to answer two questions without the help of any document. The duration of the examination is 35 minutes per student (10 minutes for the preparation of the answers, 25 minutes to present and discuss the answers). The work done during the practicals will be evaluated through a report prepared by each student. The final mark will be calculated as follows : 25% for the report on the practicals and 75% for the oral examination. | |||||
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| Contacts : | - J. Dommes, Professor : Tel. : 04/366.38.99, fax : 04/366.38.72, E-mail j.dommes@ulg.ac.be - C. Gauthier, research assistant : Tel. : 04/366.38.73, E-mail carole.gauthier@ulg.ac.be | |||||
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ULg : Students and Studies Administration - Academic Affairs
Contact : Monique Marcourt, direction A.E.E.
Date of data : 27/02/2006
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