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| BOTA0210-1

 | Plant molecular biology

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| Duration : | 30h Th, 30h Pr | |
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| Credits/ECTS : |
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| Holder(s) : | Jacques Dommes | |
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| Course contents :
| The course contains 4 parts. The two first ones deal with the bases of molecular biology (from the gene to the protein) focusing on possible differences between plants and other organisms. The third part presents the intracellular signal transduction pathways. The last part is an introduction to the main methods used in the study of gene regulation. The principle of these methods, as well as their conditions of use and their limitations, will be presented. A large number of examples are presented all along the different part of the course. This allows to apply the theoretical concepts to real problems taken from the most recent scientific literature. | |
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| Course objective :
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- To remember and to complete the theoretical bases of molecular biology.
- To get acquainted with the specificities of the plant world.
- To get acquainted with the main methods used in molecular biology.
- To apply this acquired knowledge to the study of plant development.
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| Prerequisites :
| The required background is given during the second year in the following courses : Biochemistry and thermodynamic of biological systems" and "Introduction to genetics". | |
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| Workshops :
| While applying different concepts of molecular biology, the students will mainly practise different methods described in the fourth part of the course. | |
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| Organization :
| Lectures : the timetable will be available on the first day of the academic year (Welcome address and information session at 8:30 am in the main lecture room of B-22 building). All lectures will be given in one of the lecture rooms of B-22 building. Practicals : half-day sessions in one of the student laboratories of the B-22 building. Each student should bring a white coat and a notebook. | |
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| Written notes :
| PowerPoint presentations will be available (paper copy and CD-ROM). Main reference book : - Biochemistry & Molecular Biology of Plants : B.B. Buchanan, W. Gruissem, R.L. Jones, 2000. American Society of Plant Physiologists, Rockville, USA. Other interesting books : - Gènes VI : B. Lewin, 1997. De Boeck Université, Paris, France. - Plant Biochemistry & Molecular Biology : H.W. Heldt, 1997. Oxford University Press. - Biologie Cellulaire et Moléculaire - Concepts et expériences : G. Karp, 1998. De Boeck Université. - Biologie Moléculaire de la Cellule : J. Darnel, H. Lodish, D. Baltimore, 1993. De Boeck Université. | |
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| Assessment :
| They will be an oral examination and each student will have to choose between two possibilities. In the first one, he will receive row data from a recent publication and he will be asked to describe and interpret these results, as well as to propose new experiments. He will be allowed to consult any document he wishes to prepare his answer. The second possibility is a more classical examination where the student will have to answer two questions without the help of any document. In any case the duration of the examination is 35 minutes per student (10 minutes for the preparation of the answers, 25 minutes to present and discuss the answers. The work done during the practicals will be evaluated through a report prepared by each student. The final mark will be calculated as follows : 25% for the report on the practicals and 75% for the oral examination. | |
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| Contacts :
| - J. Dommes, Professor : Tél. : 04/366.38.99, fax : 04/366.38.72, E-mail j.dommes@ulg.ac.be - C. Gauthier, research assistant : Tél. : 04/366.38.73 | |
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