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| BIOC0218-1 | Bioenergetics, partim animal | |||||
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| Duration : | 15h Th, 20h Pr | |||||
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| Holder(s) : | Francis Sluse | |||||
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| Course contents : | A) Introduction : - life, energy and metabolism - ATP and ionic gradients: intermediary stock of energy - oxygen: benefits and dangers - cofactors in redox reactions. B) Respiratory chains : - mitochondrial respiratory chain , electron flux, organization of protein complexes - bacterial respiratoty chains - chemiosmotic mechanism - energy transfer via a proton gradient. C) ATP synthesis : -stoichiometry - H+/P ratio: kinetic approach and thermodynamic approach. D) H+ electrochemical gradient : - principles of its generation - mechanisms with a transport of hydrogen - ubiquinone cycle - H+ redox pumps. E) Electron transfer mechanism. F) ATP synthesis : - energetic - enzymatic mechanism - conformational model. G) Coupling and uncoupling between respiration and phosphorylation (OXPHOS) : - coupling mechanism - uncoupling agents - difference between intinsic uncoupling and H+ gradient dissipation - compartmentation - translocators - control of ATP synthesis. H) OXPHOS : - molecular mechanisms and structures of complexes I, III, IV and V. I) Other uses of the H+ gradient : - transports of metabolites (MCF) - cation transports. J) Energy redox dissipation (plants, protists, fungi) - role - NADH dehydrogenases - alternative oxidase. K) H+ electrochemical gradient dissipation : uncoupling proteins - functional evidence - regulation - contribution to respiration - effect on OXPHOS yield. L) Interaction between energy dissipating systems via a common effector in plants : - differential expression - possible metabolic role. | |||||
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| Course objective : | Respiration generates a proton electrochemical gradient which can be used as driving force for ATP synthesis. Aims of the course are to explain the principles that govern oxidative phosphorylation and to study the structures of protein complexes implicated. The balance between energy conserving and energy dissipating systems is stressed. | |||||
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| Prerequisites : | General Biochemistry, Cellular physiology | |||||
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| Workshops : | Training in research laboratory or "live " displays or video-displays and "powerpoint" | |||||
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| Organization : | 2 lessons of 2h per week during the 1st semester | |||||
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| Written notes : | "Bioenergetics 3" by David G. Nicholls and Stuart J. Ferguson, Academic press 2002 + book chapters and articles written in English by F. Sluse | |||||
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| Assessment : | Oral exam ,4-5 students together without competition between students. | |||||
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| Contacts : | Francis E. SLUSE Laboratory of Bioenergetics, Institute of Chemistry, Bât. B6c (3rd floor ), 4000 Liège 1 (Sart Tilman). Tél. : 04/366.35.87 ou 35.71 - Fax : 04/366.28.78 - E-mail : F.Sluse@ulg.ac.be | |||||
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ULg : Students and Studies Administration - Academic Affairs
Contact : Monique Marcourt, direction A.E.E.
Date of data : 27/02/2006
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